purging hplc pump. During this step, switch the injection and the column selection valves betweenCheck if the spring in the pump head piston housing is broken . purging hplc pump

 
 During this step, switch the injection and the column selection valves betweenCheck if the spring in the pump head piston housing is broken purging hplc pump Prime the pump with degassed solvent

solvents. 02 to 0. How do i manually prime/purge it? Because there are some air bubbles, and if they get inside the needle, its gonna be bad news bears ( i think). UltiMate 3000 Series: SD, RS, BM, and BX Pumps Operating Instructions Page I Declaration of Conformity (Original Declaration of Conformity) Product: Thermo Scientific Dionex UltiMate 3000 - Pump Types: ISO-3100SD, ISO-3100BMWe have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. Cap seal for purge valve knob; Cleaning swabs; Figures. E. [][]Fill up & attach Labels of the solvent reservoirs properly. In other words is the system configuration comprising of the pump, injector/autosampler, column, and detector is appropriate for carrying out the analysis in. The technical specifications of all pumps meet the highest. Second, the dissolved gasses must diffuse through the membrane (flat film or tubing wall). (Prime each pump head separately. ), prime system if necessary. The pressure does not rise. Label bottles correctly with bottle content, and filling date / expiry date. 02 in. Pressure B. Turn the handle counterclockwise, about 1/2-turn, to open the valve, and pull back on the plunger to remove air bubbles. 17: Ready Check Results window appear s after the NC pump blocks purge is executed 2. Turn the black valve anticlockwise to purge the mobile phase. 8:42. During step 1, check that the solvent is freely moving through the solvent lines. Prime the HPLC pumps under the following conditions: - starting the pumps for the first time after it has been idle for more than 24 hours. If the column was used with buffered. a Right-click the pump icon. 5. Air bubbles Filter using a 0. The degasser is a standard feature of the 1525µ Binary HPLC Pump and an optional feature of the 1525 analytical and 1525 EF Binary HPLC Pumps. Manually prime using a priming syringe: Insert the priming syringe into the Luer fitting at the center of the draw-off valve handle. Purge pump at high flow rate (e. 05 MPa or 0. The workstation pumps will run at a default flow rate of 10 ml/min and the indicator light will flash green. OBJECTIVE: To clean and flush an Alliance HPLC System or an ACQUITY UPLC system to remove contaminants. What is pump seal in HPLC?Dionex UltiMate 3000 2G Pump Maintenance Kits vary by pump. It provides HPLC systems with an automatic, continuous method of removing dissolved gases from mobile phases. Pathology EducationNitrogen purging plays an important role in the safety and functioning of various plants that are susceptible to fire hazards. Open the purge valve on the Agilent Infinity 1260 isocratic pump b. If the solvent line is "dry," perform "dry" primes by opening the manual prime and purge valve, and draw out 10 mL from each solvent line using a syringe. b Select Set up pump. Connect its other end to a waste container. L/mni for 5mni . The pump (s) should be run (column disconnected and bypassed) run with a solution of pure HPLC grade water to try and rinse the material off. 2:32. (1ml/min) to 4 or 5 hour. 4411-EN-2. derivatization reagent, microdialysis liquid,…)The 1290 Infinity II Bio Flexible Pump is a UHPLC pump consisting of biocompatible material, designed for use in biopharma and other applications utilizing high-salt and extreme-pH conditions. Before using an HPLC system, you should prime all of the lines in your HPLC pump. Autosampler – draws samples from vials and injects them into the solvent flow provided by the pump. Leaking pump heads Check the seals and replace, if necessary. c. We have been experiencing some pressure problems on our hplc lately and the problem is mostly fluctuations in the pump head. Once the system is purged, set the flow rate at 0. Prepare the solvent: Begin by ensuring that the solvent is filtered and degassed. Press the Inject button in Empower to wait for injection. Purge each mobile phase that will be used by selecting “Purge” on the PumpModule tab on Chromeleon. DO NOT PUMP ANY FLUSHING SOLVENT THROUGH THE SEAL WASH LINE, OR INJECTION NEEDLE LINES. You basically tell it to shut down when the pressure drops to a specified level. g. Solving Problems That Matter - Bioprocessing. This installment is the first of a series of four white papers on high performance liquid chromatography (HPLC) modules (pump, autosampler, ultraviolet [UV] detector, and. 1 Turn on LC20AD (component 4) and SIL20A (component 3). Therefore, the term "the Purge pump and attempt to clean check valves. Frit, PTFE, 5/pk Add to Favorites + Create New list Item successfully added to your list Subscribe to this item in cart or checkout. Leak check: the Vice President HPLC, Fraser McLeod. If a column shows a pressure increase in a short period of use (small number of injections), there is usually an underlying problem. 1. If the preceding step does not solve the issue, remove the purge valve and start the pump. Open the "drain" on LC-20AD. If you have a pump with an automatic purge valve, read the note below the article. Replacing a Check Valve Cartridge. (Most ternary pumps actually have four solvent reservoirs. Initial investigation should consist of purging the pump with fresh eluent to confirm no air is present within the pump, as the presence of air within the pump may cause fluctuations in pressure. To wash the pump: 1. Products. Make sure that you know which solvent is linked to which pump. DO NOT PURGE THE SYSTEM WITH THE BALL VALVE CLOSED! THIS WILL RESULT IN PRESSURE TOO HIGH FOR THE PUMP TO HANDLE AND CAN RUIN THE COLUMN!!! 6. )• Operation Purging the Pump Purging the pump means rinsing the pump for a short time at a higher flow rate, using the selected solvents. To evaluate the performance of the valves make a test run, or run the Pump Leak Rate Test in the. Wash the system with isopropanol (excluding the column). High Pressure Liquid Chromatography. After the pump, the mobile phase encounters the autosampler. Purge the pump, check general functionality (for instance with a diagnostics test), read pre-compression value from pump panel and compare against values from manual (Thermo Scientific Dionex pumps only). After purging is done click [Pump on] button / to deliver the solvent for at least 20 to 30min to rinse the flow line . • After few seconds, the display will show, Flow 1. This sets the mobile phase composition to 100% water so we can purge the water line. Open the Purge Valve of your pump and purge each solvent line, one by one, with HPLC-quality Isopropanol (IPA). Algae Growth in HPLC Systems 54 Prevent Blocking of Solvent Filters 55 Normal Phase Applications 57 4 Optimizing Performance 59. Overall Routine Pump Maintenance 1. 1) Locate the pump draw-off valce (handle located on front of the pump) and priming syringe. The 1260 Infinity II Flexible Pump is a quaternary UHPLC pump that provides gradient formation and solvent blending from up to four solvents. • Mast kit with mixer, purge valves and manual injector • Pre-formed 0. Prepare the Pump • Use fresh or different mobile phase (as required). UltiMate 3000 Series: SD, RS, BM, and BX Pumps Page II Operating Instructions. 5 0. Draw approximately 20 mL of fluid. If still you have the same problem, checkout the rest of tubing. HPLC is an abbreviation for High Performance Liquid Chromatography. If the pressure does not rise, the purge valve needs to be replaced as it was the cause of high pressure. Peak shape C. This video demonstrates the proper procedure to manually prime the pump on a Shimadzu LC 40 instrument. I usually let the column drip into a beaker while washing it. The Agilent 1260 Infinity II Flexible Pump is a quaternary UHPLC system pump that provides gradient formation and solvent blending from up to four solvents. Perform flow rate check. When I used the pump C last week the pressure suddenly fell down from 300 PSI to 0 (with 100% methanol, 0. For. Priming and Purging the 1525 Binary HPLC Pump. isolating bioactive compounds the so called High Performance Liquid Chromatography (HPLC). An autosampler works by aspirating and injecting a precise sample volume into the mobile phase stream. After waiting a specified time, it starts pumping the mobile phase at the flow rate specified in the analytical method to automatically equilibrate the. • Vacuum chambers – Contain the gas-permeable eluent channel. A damping unit is connected between the two piston chambers. 5301 Stevens Creek Blvd. 1260 Infinity Binary Pump User Manual 11 Introduction to the Binary Pump 1 Instrument and OperationThe pump assembly includes a pump head with a passive inlet valve and an outlet valve. Flush the pump head with an appropriate solvent using the purge function; Turn off the pump flow and wait until the system pressure is down to zero; Analysis. The green mark will change into the A2 and B2 side 9 Repeat step 8 with the next 3 valves. 액상미립자측정기에서의 purge는 측정완료된 샘플용액을 제거하기 위해 사용하는 것 같다. HPLC Starter Kit G4201-68707 195 HPLC Starter Kit G4202-68707 195 HPLC System Tool Kit 196 Bottle Head Assembly 197 Solvent Cabinet 198. Perform search. The reduction in condensation risk can allow the pump to achieve its vapor duty at. Calculate correlation coefficient should not be less than 0. For a system in reversed phase -> Purge all lines with hot HPLC-grade water (60 to 70 °C) to remove salts or ACN polymeric by-products. (Prime each pump head separately. (2) Dry Priming using the HPLC pump to draw the mobile phase out of the bottles, through the lines, degasser channels and to the pump head or multichannel valve. Air bubbles can create such flow anomalies. Troubleshooting Strategy and Processes 5. 9 Purge a short while longer, and then close the purge valve. Then press “Enter”, “1”, “Enter”, “CE”, “CE”. The historical evolution of HPLC pumps and their characteristics has been reviewed in many textbooks (1–3), as well as journal articles (4–6). (It has been simplified for clarity. This pump also facilitates seamless method transfer using ISET and automated buffer blending. Remove and disassemble the pump head. Treatment 2:Open the compression fitting at the top of the outlet check valve with a slow pump flow until solvent leaks around the fitting. Replace PTFE frit in the purge valve. First, dissolved gasses in the mobile phase must diffuse to the surface of the membrane or film. The pressure can be monitored at your analog output signal, with the handheld controller,With the Waters Arc HPLC System, you can count on high-efficiency separations and quality data to meet your regulatory requirements with confidence. Overlay of 6 pump proportioning chromatograms in 10% gradient. 8. 2 to 0. HPLC에서의 purge는 불필요한 기체를 제거하기 위해, 2. Over time, these frits will accu. HPLC system. 6:51. Open the Purge Valve of your pump. When they fill with air, they lose their prime and stop • Effects Column Life pumping [26]. The good news is that there is a simple solution: open the purge valve, turn the flow rate to five mLs a minute, let it sit there for 10 to. Repeat twice after the cycle has finished after approximately 4 min. It can mainly divided by two types 1. HPLC Principle. A low pressure gradient (LPG) for reliable blending of up to four eluents. 3:36. c. Rough pumps stay on so the system remains under vacuum. Open prime/purge valve (in the case of Chrom Tech pumps, you turn the knob counterclockwise one to two turns. Vanquish Binary Pump C: Up to 700 bar (10,000 psi) and 10 mL/min. When launching a long and continuous analysis sequence, for example 40 samples (analysis of each sample. However, in order to obtain a full understanding of the pump, Dionex recommends that you review the manual thoroughly before beginning operation. Set a flow of 5 mL/min and purge for 5 min. 1ml/min2 g. This is needed to purge any air from the tubing, introduce fresh mobile phase to each line and then toHPLC, and biocompatible pumps. The pump assembly includes a pump head with an active inlet valve which has an replaceable cartridge, and an outlet valve. Search by lot number or partial lot number. to the reader when operating the Thermo Scientific™ Dionex™ pump. Replace the O-Ring on the Purge Valve 120 Remove the Pump Head Assembly 122 Maintenance of a Pump Head without Seal Wash 124 Maintenance of a Pump Head with Seal Wash 127• Vacuum pump – An electrical two-speed stepper motor pump that creates a vacuum in the vacuum chambers. This post continues the series of Chromatography Explained and will focus on introducing HPLC. Inside the purge valve is a small piece of polytetrafluoroethylene, called a PTFE frit. The name of the game in HPLC is repeatability. You. Preparing the Binary Pump 51 Algae Growth in HPLC Systems 52 Setting up the Pump with the Instrument Control Interface 54 Priming the Pump 64 Solvent Information 66. Air bubbles can create such flow anomalies. Pump a 20% nitric acid/water solution through the pump for 3 minutes. SPE, LLE) on. If using MassLynx or Empower. Solvent mixing: The proportionating valve (lower-front of image) allows the desired volume ratio of the different solvents to be drawn from the reservoirs. A damping unit is connectedThe 1260 Infinity II Quaternary Pump is a gradient pump that uses low-pressure mixing for UHPLC gradient formation and solvent blending from up to four solvents. Liquid Chromatography (LC) Troubleshooting Resources. 2 Content 4 2. Weigh the sample into a syringe. g. Irreproducible gradient. The 1290 Infinity II Flexible Pump is a quaternary pump that provides UHPLC gradient formation and solvent blending from up to four solvents. Consumables likely need to be replaced on aqueous pump. Run at least 25 ml buffer through degasser and pump (5 minutes) e. Page 70 Using the Pump Algae Growth in HPLC Systems frits to block over short-term usage. 1 Turn on LC20AD (component 4) and SIL20A (component 3). Algae Growth in HPLC Systems 53 5 Optimizing Performance 55. 2 ml/min) for a moment and than went back to 300. Start pump purging (all channels, including those not used for the application) and wait until pump purging is complete; Flush the entire chromatography system with the starting solvent of your application at a reasonable flow rate to rinse out any other solvent; Install the appropriate HPLC column for your application; Close all doors of the. Place the solvent bottle back into LC eluent. the user. 1–1% solution by volume is sufficient. 02 in. purge를 실행하기 전에 꼭 DRAIN 밸브를 열어주고 실행하며, purge가 끝난 후 밸브를 다시 닫아줍니다. Moreover, if the faulty purge valve completely blocks the engine, it cannot start or run. The user manual for the Agilent G1311A Quaternary Pump, a high-performance liquid chromatography (HPLC) module that provides reliable gradient formation and precise flow rates. 4. Read this guide thoroughly before using the product and operate the product in accordance with the instructions in this manual. Relevant functions marked for purging, flow settings ect. e. You may. 1 Overview of HPLC. Prepare the Sampler • Avoid using needle wash and seat backflush with organic solvents with buffer applications, Most solvents need to be degassed, so U/HPLC systems typically include a solvent degassing system. Prime the pump with degassed solvent. Set the pump to 100% 'A' at 1. Changing from one operating pump head to the other you simply move the yellow connection tube to the other pump head outlet. Then wash them out with water and re-assemble them on the pump head. Mass spectrometry, chromatography, spectroscopy, software, dissolution, sample handling and vacuum technologies coursesRemedy. Gradually increase system flow up to the required flow rate,. Retention. Agilent recommends a purge of at least 30mL in total for the 1200 systems. 1-15 of 16. 6. Liquid Chromatography. Peaks Peak area precision Pressure Retention times Baseline Troubleshooting overview Running into issues with your HPLC or UHPLC analysis?8th May 2023 HPLC Pump Care and Troubleshooting Many of the technical support inquiries we receive at Chrom Tech are related to care and troubleshooting of our. Their pulse-free solvent flow, from analytical to semi-preparative flow rates, makes them ideal for use with highly sensitive detectors. For reverse-phase columns, wet the column using a mobile phase like methanol and bring it to normal flow rate. 5 kgfcm -2, for example) for 10 to 30 minutes to purge the air from the mobile phase. It is column chromatography. Figure 1 Overview of the binary pump Purge valve Active inlet valve Mixer Outlet valve. a Continue to purge for a short while. The auto purge function was achieved by devising the mechanism of flow lines including the high-pressure 6-way valve and by modifying the solvent delivery unit check valve. Evaluate the results; Stop the batch; Apply a corrective pump purge; Re-equilibrate the system; Restart the batch Nexera looks for evidence of flow. Disconnect tubing at guard column (if present) or analytical column inlet. A peristaltic pump (piezo pump) delivers fresh wash solvents to the fl ush port. ), prime system if necessary. Is the pump fuse in working order?Discover faster analysis, better resolution, and more reproducible chromatography with our new flagship UHPLC pump. b. Use the keypad to type in the solvent composition for pumps A, B, C, and D. Priming and Purging the 1525 Binary HPLC Pump. Then wash them out with water and re-assemble them on the pump head. Close the Purge Valve and flush the system. Versatile and Flexible The Thermo Scientific™ Dionex™ UltiMate 3000 pump family offers the most complete choice in the industry. Priming and Purging the 1525 Binary HPLC Pump. After waiting a specified time, it starts pumping the mobile phase at the flow rate specified in the analytical method to automatically equilibrate the. Remove and clean pistons. 9. Monitoring your HPLC system for potential issues can help to. Open the Purge Valve by turning it counterclockwise b. 0 • November 2022 *4820. For more information on purging the pump, see the reference. ) If system has check valve, loosen valve to allow air to escape. • Future-proof flow-pressure footprint provides highest application flexibility. Initial System inspection It is always worthwhile to spend a few minutes before starting analysis on assessing the system suitability for the analysis task in hand. e. close the purge. 5. Choose between 3 configurations: A 2 × 2 binary high pressure gradient (HPG) for high accuracy blending of up to two eluents from two selectable solvents each. Set a flow of 5 ml/min and purge each channel for 5 min with HPLC-quality Water. ), prime system if necessary. HPLC is an abbreviation for High Performance Liquid Chromatography. 6 Purging the Pump. When the purge valve is open, solvent from the quaternary pump will go straight to waste rather than proceeding through the column. Try adding acid or base to the wash solvent to see if the carryover is improved. It is Liquid Chromatography. The stationary phase is a granular material with very small porous particles in a separation column. between the degasser and the column. Push the “PURGE” button on the front of the pump. operating pressure (9,99 ml/min max. In stainless steel (SS) the passivation process uses nitric acid or citric acid to remove free iron from the surface. It also enables quaternary solvent delivery at. After purging the pump heads, pu rge the ProFlow flow meter for about 10 minutes. This pump enables quaternary solvent delivery at pressures up to 800. Fig. Reducing the diffusion path to improve degassing. 7. Run through the prime purge valve first (10 mls), then. Seal could have fluoropolymer O-ring energizer instead (black O-ring). Afterwards. If the pressure drops off suddenly (by more than a few kgf/cm 2), the backpressure tube is clogged. 7. OBJECTIVE: To clean and flush an Alliance HPLC System or an ACQUITY UPLC system to remove contaminants. Purging has only helped to reduce. Achieve binary-like performance in your HPLC instrument. |The 1290 Infinity II Bio Flexible Pump. Agilent University. Page 103 • Operation Figure 29: Dwell volume. Turn the handle counterclockwise, about 1/2-turn, to open the valve, and pull back on the plunger to remove air bubbles. • Purge each channe lwtih 25–3m. If the VP button does not show mobile phase, press “CE” followed by “Function” until “System” is shown on the screen. If you see a major leak from the bottom of the pump head or in the gap between the two pump head, please contact your local Agilent support representative. off. Seat backfl ush is enabled by a second pump, an integrated high-pressure fl ush pump. the solvent flow of the HPLC If equipped with a ballast valve check that the ballast valve on the rough pump is . Worldwide Emails . Before using an HPLC system, you should prime all of the lines in your HPLC pump. ⬇️ Get Affordable and Dope Lab Consumables Here ⬇️(No pun intended, unless you're a cannabis lab, then pun intended)to Connect with. Operable in constant flow or constant pressure mode, as well as isocratic, low pressure gradient, ternary high-pressure gradient, or quaternary low. - For Quick Start: Click the "Press to make a single injection" button in the Acquisition. Load a Method a. Achieve binary-like performance in your HPLC instrument. For best performance you must know your eluents 2. Some pumps will require to manually open the purge valve and in others it is done via the software. Open the black purge valve and press the “Purge” button. ( refer to Pump Operating Manual ). Properly shut down all the HPLC modules from the front (LC-20 and LC-30) or backUltiMate 3000 Series: SD, RS, BM, and BX Pumps Operating Instructions Page I Declaration of Conformity (Original Declaration of Conformity) Product: Thermo Scientific Dionex UltiMate 3000 - Pump Types: ISO-3100SD, ISO-3100BMThe PTFE frits in your Agilent LC pumps will need to be replaced from time to time as part of a routine maintenance program. Autosampler – draws samples from vials and injects them into the solvent flow provided by the pump. A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pump head. The dual piston design has minimal pulsation for stable baselines. thermoscientific Vanquish Pump H VH-P10-A-02 Operating Manual 4820. Honoring the Past, Focusing on the Future: A Conversation with Jess Lee. Remove sample vials from the auto-sampler tray g. In fire and explosion protection engineering, an inert (ie, non-flammable) purge gas (like nitrogen, helium, argon, etc) is introduced into an enclosed system (eg, a container or process vessel) to prevent the formation of a fuming. Make sure that the AVR7-3 inject valve is in the Purge position. Purge pump at high flow rate (e. The following diagrams illustrate the operating principle of a modern HPLC pump: an electric motor drives a piston (generally made of sapphire or ceramic material) in and out of metal, ceramic, or PEEK piston chamber. 19 Section 3: Pump Maintenance 20 Purge Valve 21 Outlet Ball Valve 21 Active Inlet Valve (AIV) 22 Seals and Pistons 25 Pump Head 26 Parts and Supplies 27 Solvent Inlet FiltersLC-40D XR Specifications. Waters Second Quarter 2023 Highlights. Press “Menu” till the purge appears on the screen. 8. If problem persists, flush system with 100% methanol or isopropanol. 1, 3, and 4. 5 mL/min d. Solvent Degasser – removes air gases from the solvents as they flow to the HPLC pump. With an unrivaled flow-pressure footprint, the RSLC pump is the most flexible binary UHPLC pump available. Press the purge buttons again to. Passivation is a metal finishing process to prevent corrosion and resist rust. (Prime each pump head separately. 01. Worldwide Numbers . The sensor signals the control board when the vacuum is below a preset level. • Outstanding reliability and durability combined with unrivaled flow and gradient performance. Email Priming and Purging a 1525 Binary Series HPLC Pump to a friend ; Read More. The reduction in condensation risk can allow the pump to achieve its vapor duty at. Set the flow rate to 5 ml/min d. The target url is a user manual for the Agilent 1290 Infinity Quaternary Pump G4204A, a high-performance liquid chromatography (HPLC) device that can handle a wide range of flow rates and compositions. 물론 이건 내 생각이다! 여튼 purge. 00 ml/min, turn the pump back ON and close the prime purge valve to allow the liquid to flow through solvent lines, through the degasser channel, through the pump. It is Liquid Chromatography. At the extreme, air in the pump will cause the pump to stop delivering mobile phase to the column. It offers gradient formation from up to four solvents at pressures up to 1300 bar and flow rates up to 5 mL/min. However, even if the degassing system works properly, some air bubbles might stick in solvent lines or U/HPLC system fluidics. 5. HPLC Pump – provides solvent flow and proportioning. Changing the Purge Valve Frit/Purge Valve for Binary Pumps. Sonication: ultrasonic baths as a stand-alone. Channels in column Replace column. 1 M is considered 'high', but all buffers should be flushed out) can damage the pump pistons, pump seals, injector. Confirm that the lever is in the INJECT position, insert the syringe all the way into the injection port, and quickly switch the valve to the LOAD position. Kata kunci / keywords: HPLC/HPLC instrument, metode analisis/analysis method, metode isolasi/ isolation method, bahan kimia alam/ natural chemical product. Check for flow. 2 Set the flow of the pump at 1ml/min. The new 1290 Infinity II LC power range has a high instrument efficiency, allowing you to run any HPLC and UHPLC method. RCM (right click mouse) in the Quat Pump module i. This video explains the reasons for pump priming. 9 Pump Types LPG pump type Eluent composition at the low pressure side => Before the pump head Eluent is composed through proportioning valves Eluent segments pass working and equilibration cylinder Up to four solvents can be mixed HPG pump type Eluent composition at the high pressure side => Behind the pump head Pure solvents pass. If you have an Agilent 1260 Infinity Isocratic or Quaternary Pump, remove the tubing to your passive inlet valve (PIV). HPLC에서의 purge는 불필요한 기체를 제거하기 위해, 2. The two channels are fed into a low-volume mixing chamber which is connected via a restriction capillary coil to a damping unit and a mixer. The flow diagram to the right explains this function. For GPC columns, remove column from the flow path so nothing is accidently flushed into the column. - filter non-HPLC grade solvents - filter buffer solutions - Install an in-line filter between auto-sampler and column (removes pump seal debris, ALS rotor debris, and sample particulates). 물론 이건 내 생각이다! 여튼 purge. - Troubleshooting the degasser always needs to be linked with troubleshooting the mobile phase. This guide describes the basic operation and troubleshooting for the ExionLCTM AD system . Close the purge valve, set the flow to a value that gives a pressure of 350 bar. Algae Growth in HPLC Systems 81 Prevent Blocking of Solvent Filters 82 Normal Phase Applications 83 4 Optimizing Performance 85 Using the Degasser 86. then you have a little bit of work to do to really prime the line as that pump is not really powerful enough to draw in liquid if it is super dry. Need to change the pump seal on your Shimadzu LC-20AD HPLC? Boom, I got you. General Recommendations For effi cient reduction of carryover, it is important that the sample be soluble in weak and strong wash solvents.